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- PD-1 (CD279) Antibodies
Invitrogen
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说明书
实验方案
技术支持
Cite CD279 (PD-1) Monoclonal Antibody (RMP1-30), Brilliant Violet™ 421, eBioscience™
说明书
实验方案
技术支持
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CD279 (PD-1) Antibody (404-9981-82) in Flow
Swiss Webster mouse splenocytes were unstimulated (left) or stimulated for 72 hours with CD3e and CD28 Monoclonal Antibodies, Functional Grade (Product # 16-0031-85) and (Product # 16-0281-85) (right). Cells were then stained with 1.0 µg of Rat IgG2b kappa Isotype Control, Brilliant Violet 421 (Product # 404-4031-81) (blue histogram) or 1.0 µg of CD279 (PD-1) Monoclonal Antibody, Brilliant Violet 421 (purple histogram). Total viable cells were used for analysis, as determined by 7-AAD (Product # 00-6993-50). {{ $ctrl.currentElement.advancedVerification.fullName }} 验证信息 View more
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产品信息
404-9981-82
应用
建议稀释比
已发表文章
流式细胞分析(Flow)
1.0 µg/test
-
产品规格
种属反应
Mouse
宿主/亚型
Rat/ IgG2b, kappa
推荐的同型对照
Rat IgG2b kappa Isotype Control (eB149/10H5), Brilliant Violet™ 421, eBioscience™
分类
Monoclonal
类型
Antibody
克隆号
RMP1-30
偶联物
Brilliant Violet™ 421
- APC
- Biotin
- eFluor 450
- FITC
- PE
- PerCP-eFluor 710
- Super Bright 436
- Super Bright 600
- Unconjugated
- Request custom conjugation
激发/发射光谱
406/423 nm查看光谱
形式
liquid
浓度
0.2 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2, with BSA
内含物
0.09% sodium azide
保存条件
4° C, store in dark, DO NOT FREEZE!
运输条件
Wet ice
产品详细信息
Description: The RMP1-30 antibody reacts with mouse PD-1 (programmed death-1), a 55 kDa member of the Ig superfamily. PD-1 contains the immunoreceptor tyrosine-based inhibitory motif (ITIM) and plays a key role in peripheral tolerance and autoimmune disease in mice. PD-1 is expressed mainly on activated T and B lymphocytes. Two novel B7 Family members have been identified as PD-1 ligands, PD-L1 (B7-H1) and PD-L2 (B7-DC). Evidence reported to date suggests overlapping functions for these ligands and their constitutive expression on some normal tissues and upregulation on activated antigen-presenting cells. RMP1-30 does not block the binding of either B7-H1-Ig or B7-DC-Ig to PD-1 transfectants.
Applications Reported: This RMP1-30 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This RMP1-30 antibody has been tested by flow cytometric analysis of mouse splenocytes. This may be used at less than or equal to 1.0 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Brilliant Violet™ 421 (BV421) is a dye that emits at 423 nm and is intended for use on cytometers equipped with a violet (405 nm) laser. Please make sure that your instrument is capable of detecting this fluorochrome.
When using two or more Super Bright, Brilliant Violet™, Brilliant Ultra Violet™, or other polymer dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401-42) or Brilliant Stain Buffer™ (Product # 00-4409-75) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer or Brilliant Stain Buffer for more information.
Excitation: 407 nm; Emission: 423 nm; Laser: Violet Laser.
BRILLIANT VIOLET™ is a trademark or registered trademark of Becton, Dickinson and Company or its affiliates, and is used under license. Powered by Sirigen.™
靶标信息
Cell-mediated immune responses are initiated by T lymphocytes that are themselves stimulated by cognate peptides bound to MHC molecules on antig en-presenting cells (APC). T-cell activation is generally self-limited as activated T cells express receptors such as PD-1 (also known as PDCD-1) that mediate inhibitory signals from the APC. PD-1 can bind two different but related ligands, PDL-1 and PDL-2. Upon binding to either of these ligands, signals generated by PD-1 inhibit the activation of the immune response in the absence of "danger signals" such as LPS or other molecules associated with bacteria or other pathogens. Evidence for this is seen in PD1-null mice who exhibit hyperactivated immune systems and autoimmune diseases. Despite its predicted molecular weight, PD-1 often migrates at higher molecular weight in SDS-PAGE.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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引用该产品
生物信息学
蛋白别名:CD279; hPD1; mPD-1; Programmed cell death protein 1; Protein PD-1; Protein PD1; sCD279; soluble CD279
UniProt ID:(Mouse) Q02242
Entrez Gene ID:(Mouse) 18566
功能
protein binding
参与通路
immune system processnegative regulation of tolerance inductionapoptotic processpositive regulation of apoptotic processnegative regulation of apoptotic processpositive regulation of T cell apoptotic process
It has to be done as per old AB suggested Products section.